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Western blot analysis of PPARGC1A in mouse liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.
Immunohistochemistry of PPARGC1A in human liver tissue with PPARGC1A antibody at 5 μg/mL.
Immunofluorescence of PPARGC1A in human liver tissue with PPARGC1A antibody at 20 μg/mL.
Western blot analysis of PPARGC1A expression in human liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of PPARGC1A in mouse liver tissue lysate with PPARGC1A antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

PPARGC1A Antibody

CATALOG NUMBER: 7705

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Homology:
Predicted species reactivity based on immunogen sequence: Pig: (100%), Bovine: (100%)
Immunogen:
PPARGC1A antibody was raised against a 19 amino acid peptide near the carboxy terminus of human PPARGC1A.
The immunogen is located within the last 50 amino acids of PPARGC1A.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PPARGC1A antibody can be used for detection of PPARGC1A by Western blot at 1 - 2 μg/ml. Antibody can also be used for Immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human and mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PPARGC1A antibody is human, mouse and rat reactive. At least three isoforms of PPARGC1A are known to exist; this antibody only detects the longest isoform.
Positive Control 1:
Cat. No. 1404 - Mouse Liver Tissue Lysate
Positive Control 2:
Cat. No. 10-201 - Human Liver Tissue Slide
Positive Control 3:
Cat. No. 1304 – Human Liver Tissue Lysate
Predicted Molecular Weight:
Predicted: 90 kDa
Observed: 88 kDa
Purification:
PPARGC1A antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPARGC1A antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPARGC1A antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPARGC1A
Additional Names:
PPARGC1A Antibody: LEM6, PGC1, PGC1A, PGC-1v, PPARGC1, PGC-1(alpha), LEM6, Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, Ligand effect modulator 6, PGC-1-alpha
Protein Accession Number:
NP_037393
Protein Gi Number:
7019499
Ncbi Gene Id Number:
10891
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The peroxisome proliferator-activated receptor gamma coactivator 1-alpha (PPARGC1A), also known as LEM-6, is a transcriptional coactivator that regulates the genes involved in energy metabolism (1). PPARGC1A interacts with PPARgamma, which permits the interaction of PPARGC1A with multiple transcription factors. PPARGC1A can interact with, and regulate the activities of, cAMP response element binding protein (CREB) and nuclear respiratory factors (NRFs). It provides a direct link between external physiological stimuli and the regulation of mitochondrial biogenesis, and is a major factor that regulates muscle fiber type determination (2). PPARGC1A may be also involved in the development of obesity (3).
Background Reference 1:
Tsuemi T and La Spada AR. PGC-1a at the intersection of bioenergetics regulation and neuron function: from Huntington's disease to Parkinson's disease and beyond. Prog. Neurobiol. 2012; 97:142-51.
Background Reference 2:
Kang C and Li Ji L. Role of PGC-1a signaling in skeletal muscle health and disease. Ann. NY Acad. Sci. 2012; 1271:110-7.
Background Reference 3:
Liu C and Lin JD. PGC-1 coactivators in the control of energy metabolism. Acta Biochim. Biophys. Sin. 2011; 43:248-57.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7705

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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