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Western blot analysis of extracts of various cell line, using PPARGC1B antibody (16-896) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 5min.
Western blot analysis of extracts of various cell line, using PPARGC1B antibody (16-896) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 5min.

PPARGC1B Antibody

CATALOG NUMBER: 16-896

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic peptide corresponding to a sequence within amino acids 850-950 of human PPARGC1B (NP_001166170.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
HeLa
Positive Control 2:
MCF7
Positive Control 3:
mouse brain
Predicted Molecular Weight:
Observed: 113kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPARGC1B
Additional Names:
ERRL1, PERC, PGC-1(beta), PGC1B, peroxisome proliferator-activated receptor gamma coactivator 1-beta, PGC-1-related estrogen receptor alpha coactivator, PPAR-gamma coactivator 1-beta, PPARGC-1-beta, PPARgamma coactivator 1 beta, peroxisome proliferator-activated receptor gamma, coactivator 1 beta
Ncbi Gene Id Number:
133522
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene stimulates the activity of several transcription factors and nuclear receptors, including estrogen receptor alpha, nuclear respiratory factor 1, and glucocorticoid receptor. The encoded protein may be involved in fat oxidation, non-oxidative glucose metabolism, and the regulation of energy expenditure. This protein is downregulated in prediabetic and type 2 diabetes mellitus patients. Certain allelic variations in this gene increase the risk of the development of obesity. Three transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-896

List Size:
100 uL

List Price:

$419.00

Out of stock

Datasheet
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