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Antibody used in IHC on Human kidney.
Antibody used in WB on Human HepG2 at 0.25 ug/ml.
Antibody used in WB on Human, Hamster at 1:5000.
Antibody used in IHC on Human kidney.

PPAT Antibody

CATALOG NUMBER: 30-151

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PPAT.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PPAT antibody can be used for detection of PPAT by ELISA at 1:62500. PPAT antibody can be used for detection of PPAT by western blot at 0.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1211 - HepG2 Cell Lysate
Predicted Molecular Weight:
56 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PPAT antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PPAT
Additional Names:
PPAT, ATASE, GPAT, PRAT
Protein Accession Number:
NP_002694
Protein Gi Number:
29570798
Ncbi Gene Id Number:
5471
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPAT is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. Its gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.The protein encoded by this gene is a member of the purine/pyrimidine phosphoribosyltransferase family. This protein is a regulatory allosteric enzyme that catalyzes the first step of de novo purine nucleotide biosynthesis. This gene and PAICS/AIRC, a bifunctional enzyme catalyzing steps six and seven in the purine nucleotide biosynthesis pathway, are located in close proximity on chromosome 4.
Background Reference 1:
Bera, A.K., (1999) J. Biol. Chem. 274 (51), 36498-36504.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-151

List Size:
100 ul

List Price:

$519.00

Datasheet
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