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Western blot analysis of PPFIA3 in 293 cell lysate with PPFIA3 antibody at (A) 1 and (B) 2 μg/ml.
Western blot analysis of PPFIA3 in 293 cell lysate with PPFIA3 antibody at (A) 1 and (B) 2 μg/ml.
Trial Size

PPFIA3 Antibody

CATALOG NUMBER: 7561

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PPFIA3 antibody was raised against a 17 amino acid peptide near the carboxy terminus of human PPFIA3.
The immunogen is located within amino acids 1100 - 1150 of PPFIA3.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PPFIA3 antibody can be used for detection of PPFIA3 by Western blot at 1 - 2 μg/ml.
Antibody validated: Western Blot in human samples. All other applications and species not yet tested.
Specificity:
PPFIA3 antibody is human, mouse, and rat reactive.
Positive Control 1:
Cat. No. 1210 - HEK293 Cell Lysate
Predicted Molecular Weight:
Predicted: 131 kDa
Observed: 131 kDa
Purification:
PPFIA3 antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPFIA3 antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPFIA3 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PPFIA3
Additional Names:
PPFIA3 Antibody: LPNA3, KIAA0654, Liprin-alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, PTPRF-interacting protein alpha-3
Protein Accession Number:
NP_003651
Protein Gi Number:
32189362
Ncbi Gene Id Number:
8541
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The PPFIA3 protein is a member of the LAR protein-tyrosine phosphatase-interacting protein (liprin) family, a family of transmembrane protein tyrosine phosphatases known to be important for axon guidance and mammary gland development (1). Similar to the other liprin family members, PPFIA3 can interact with ERC2, a member of the ELKS-Rab6-interacting protein-CAST family of proteins at the presynaptic termini, suggesting that these proteins may play a role in the molecular organization of presynaptic zones (2).
Background Reference 1:
Serra-Pages C, Medley QG, Tang M, et al. Liprins, a family of LAR transmembrane protein-tyrosine phosphatase-interacting proteins. J. Biol. Chem. 1998; 273:15611-20.
Background Reference 2:
Ko J, Na M, Kim S, et al. Interaction of the ERC family of RIM-binding proteins with the liprin-alpha family of multidomain proteins. J. Biol. Chem. 2003; 278:42377-85.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

7561

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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