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Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.
Western Blot at 1:2000 dilution Lane 1: HepG2 whole cell lysate Lane 2: Jurkat whole cell lysate Lysates/proteins at 20 ug per lane.

PPFIBP1 Antibody

CATALOG NUMBER: 59-212

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PPFIBP1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 588-617 amino acids from the Central region of human PPFIBP1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:2000
Predicted Molecular Weight:
114 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPFIBP1
Additional Names:
Liprin-beta-1, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein-binding protein 1, PTPRF-interacting protein-binding protein 1, hSGT2, PPFIBP1, KIAA1230
Protein Accession Number:
Q86W92
Protein Gi Number:
90185247
Ncbi Gene Id Number:
8496
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the LARprotein-tyrosine phosphatase-interacting protein (liprin) family.Liprins interact with members of LAR family of transmembraneprotein tyrosine phosphatases, which are known to be important foraxon guidance and mammary gland development. It has been proposedthat liprins are multivalent proteins that form complex structuresand act as scaffolds for the recruitment and anchoring of LARfamily of tyrosine phosphatases. This protein was found to interactwith S100A4, a calcium-binding protein related to tumorinvasiveness and metastasis. In vitro experiment demonstrated thatthe interaction inhibited the phosphorylation of this protein byprotein kinase C and protein kinase CK2. Alternatively splicedtranscript variants encoding distinct isoforms have been reported.
Background Reference 1:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 2:
Norrmen, C., et al. Blood 115(4):906-909(2010)
Background Reference 3:
Ewing, R.M., et al. Mol. Syst. Biol. 3, 89 (2007) :
Background Reference 4:
Olsen, J.V., et al. Cell 127(3):635-648(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-212

List Size:
400 ul

List Price:

$495.00

Datasheet
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