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Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue reacted with PPID Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of k562 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PPID using rabbit polyclonal PPID Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPID gene.
Western blot analysis of PPID Antibody in NIH-3T3 cell line lysates (35ug/lane)

PPID Antibody

CATALOG NUMBER: 63-645

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PPID antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 85-115 amino acids from the N-terminal region of human PPID.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
41 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPID
Additional Names:
Peptidyl-prolyl cis-trans isomerase D, PPIase D, 40 kDa peptidyl-prolyl cis-trans isomerase, Cyclophilin-40, CYP-40, Cyclophilin-related protein, Rotamase D, PPID, CYP40, CYPD
Protein Accession Number:
Q08752
Protein Gi Number:
729274
Ncbi Gene Id Number:
5481
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPIases accelerate the folding of proteins. It catalyzes the cis-trans isomerization of proline imidic peptide bonds in oligopeptides.
Background Reference 1:
Mayya V.,et.al., Sci. Signal. 2:RA46-RA46(2009).
Background Reference 2:
Gevaert K., et.al., Nat. Biotechnol. 21:566-569(2003).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-645

List Size:
400 ul

List Price:

$495.00

Datasheet
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