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Western blot analysis in U937 cell line lysates (35ug/lane).
Western blot analysis in U937 cell line lysates (35ug/lane).

PPIE Antibody

CATALOG NUMBER: 59-279

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This PPIE antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 22-49 amino acids from the N-terminal region of human PPIE.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
33 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPIE
Additional Names:
Peptidyl-prolyl cis-trans isomerase E, PPIase E, Cyclophilin E, Cyclophilin-33, Rotamase E, PPIE, CYP33
Protein Accession Number:
Q9UNP9
Protein Gi Number:
13124097
Ncbi Gene Id Number:
10450
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of thepeptidyl-prolyl cis-trans isomerase (PPIase) family. PPIasescatalyze the cis-trans isomerization of proline imidic peptidebonds in oligopeptides and accelerate the folding of proteins. Thisprotein contains a highly conserved cyclophilin (CYP) domain aswell as an RNA-binding domain. It was shown to possess PPIase andprotein folding activities, and it also exhibits RNA-bindingactivity. Alternative splicing results in multiple transcriptvariants. A related pseudogene, which is also located on chromosome1, has been identified.
Background Reference 1:
Park, S., et al. Biochemistry 49(31):6576-6586(2010)
Background Reference 2:
Hom, R.A., et al. J. Mol. Biol. 400(2):145-154(2010)
Background Reference 3:
Wang, Z., et al. Cell 141(7):1183-1194(2010)
Background Reference 4:
Naukkarinen, J., et al. PLoS Genet.(6), E1000976 (2010) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-279

List Size:
400 ul

List Price:

$495.00

Datasheet
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