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Western blot analysis in T47D cell line lysates (35ug/lane).
PPL Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human skin tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in T47D cell line lysates (35ug/lane).

PPL Antibody

CATALOG NUMBER: 57-532

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PPL antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 1301-1330 amino acids from the C-terminal region of human PPL.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
205 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPL
Additional Names:
Periplakin, 190 kDa paraneoplastic pemphigus antigen, 195 kDa cornified envelope precursor protein, PPL, KIAA0568
Protein Accession Number:
O60437
Ncbi Gene Id Number:
5493
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a component ofdesmosomes and of the epidermal cornified envelope inkeratinocytes. The N-terminal domain of this protein interacts withthe plasma membrane and its C-terminus interacts with intermediatefilaments. Through its rod domain, this protein forms complexeswith envoplakin. This protein may serve as a link between thecornified envelope and desmosomes as well as intermediatefilaments. AKT1/PKB, a protein kinase mediating a variety of cellgrowth and survival signaling processes, is reported to interactwith this protein, suggesting a possible role for this protein as alocalization signal in AKT1-mediated signaling. [provided byRefSeq].
Background Reference 1:
Pinheiro, A.P., et al. Am. J. Med. Genet.Neuropsychiatr. Genet. 153B (5), 1070-1080 (2010) :
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Pyle, A.L., et al. Cardiovasc. Pathol. 19 (2), E13-E20 (2010) :
Background Reference 4:
Huang, Y., et al. Arch. Dermatol. Res. 301(10):703-709(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-532

List Size:
400 ul

List Price:

$495.00

Datasheet
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