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Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.
Immunohistochemistry of PPM1D in human lung carcinoma tissue with PPM1D antibody at 5 μg/ml.
Immunofluorescence of PPM1D in human lung carcinoma tissue with PPM1D antibody at 20 μg/ml.
Western blot analysis of PPM1D in 3T3 cell lysate with PPM1D antibody at 1 μg/ml.
Trial Size

PPM1D Antibody

CATALOG NUMBER: 8043

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PPM1D antibody was raised against an 18 amino acid peptide near the carboxy terminus of human PPM1D.
The immunogen is located within the last 50 amino acids of PPM1D.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PPM1D antibody can be used for detection of PPM1D by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in mouse samples; Immunohistochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
PPM1D antibody is human, mouse and rat reactive. At least two isoforms of PPM1D are known to exist; this antibody will only detect the larger isoform.
Positive Control 1:
Cat. No. 1212 - 3T3 Cell Lysate
Positive Control 2:
Cat. No. 10-101 - Human Lung Tissue Slide
Predicted Molecular Weight:
Predicted: 67 kDa
Observed: 68 kDa
Purification:
PPM1D antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PPM1D antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PPM1D antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
Ncbi Official Symbol:
PPM1D
Additional Names:
Protein phosphatase Mg2+/Mn2+ dependent 1D, p53-induced protein phosphatase 1, PP2C-DELTA, Protein phosphatase 1D, WIP1
Protein Accession Number:
NP_003611
Protein Gi Number:
4505997
Ncbi Gene Id Number:
8493
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Wild-type p53 induced phosphatase 1 (WIP1) / protein phosphatase magnesium-dependent 1 delta (PPM1D), a protein identified in the p53 DNA response pathway, is a member of the PP2C family of serine/threonine protein phosphatases which selectively inactivates p38 MAPK and dephosphorylates the ATM/ATR targets, Chk1 and p53 (1,2). Studies have shown that it is overexpressed in human cancers and is involved in the regulation of multiple DNA damage signaling pathways (2,3). PPM1D deletion results in a reduction of T and B cell function and compromised cell division, rendering cells resistant to becoming cancerous and slowing tumor development (4,5).
Background Reference 1:
Fiscella M, Zhang H, Fan S, et al. Wip1, a novel human protein phosphatase that is induced in response to ionizing radiation in a p53-dependent manner. Proc. Natl. Acad. Sci. USA. 1997; 94:6048-53.
Background Reference 2:
Zhu YH and Bulavin DV. Wip1-dependent signaling pathways in health and diseases. Prog. Mol. Biol. Transl. Sci. 2012; 106:307-25.
Background Reference 3:
Lu X, Nguyen TA, Moon SH, et al. The type 2C phosphatase Wip1: an oncogenic regulator of tumor suppressor and DNA damage response pathways. Cancer Metastasis Rev. 2008; 27:123-35.
Background Reference 4:
Liang C, Guo E, Lu S, et al. Over-expression of wild-type p53-induced phosphatase 1 confers poor prognosis of patients with gliomas. Brain Res. 2012; 1444:65-75.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

8043

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

$65 Standard Overnight flat rate for as many products shipped within the United States.

$35 Dry Ice Shipment fee may be required on some items.

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