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Western blot analysis in MCF-7 cell line lysates (35ug/lane).
Western blot analysis in MCF-7 cell line lysates (35ug/lane).

PPM1F Antibody

CATALOG NUMBER: 60-805

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PPM1F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 275-303 amino acids from the Central region of human PPM1F.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
50 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPM1F
Additional Names:
Protein phosphatase 1F, Ca(2+)/calmodulin-dependent protein kinase phosphatase, CaM-kinase phosphatase, CaMKPase, Partner of PIX 2, Protein fem-2 homolog, hFem-2, PPM1F, KIAA0015, POPX2
Protein Accession Number:
P49593
Protein Gi Number:
24638458
Ncbi Gene Id Number:
9647
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a member of the PP2Cfamily of Ser/Thr protein phosphatases. PP2C family members areknown to be negative regulators of cell stress response pathways.This phosphatase can interact with Rho guanine nucleotide exchangefactors (PIX), and thus block the effects of p21-activated kinase 1(PAK), a protein kinase mediating biological effects downstream ofRho GTPases. Calcium/calmodulin-dependent protein kinase II gamma(CAMK2G/CAMK-II) is found to be one of the substrates of thisphosphatase. The overexpression of this phosphatase or CAMK2G hasbeen shown to mediate caspase-dependent apoptosis. An alternativelyspliced transcript variant has been identified, but its full-lengthnature has not been determined.
Background Reference 1:
Harvey, B.P., et al. J. Biol. Chem. 279(23):24889-24898(2004)
Background Reference 2:
Koh, C.G., et al. Curr. Biol. 12(4):317-321(2002)
Background Reference 3:
Tan, K.M., et al. J. Biol. Chem. 276(47):44193-44202(2001)
Background Reference 4:
Kitani, T., et al. J. Biochem. 125(6):1022-1028(1999)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

60-805

List Size:
400 ul

List Price:

$495.00

Datasheet
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