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Western blot analysis of extracts of various cell lines, using PPM1K antibody (16-674) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Western blot analysis of extracts of various cell lines, using PPM1K antibody (16-674) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 1s.

PPM1K Antibody

CATALOG NUMBER: 16-674

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 183-372 of human PPM1K (NP_689755.3).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:2000
Positive Control 1:
A-431
Positive Control 2:
Mouse heart
Positive Control 3:
Mouse brain
Positive Control 4:
Rat heart
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 41kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPM1K
Additional Names:
BDP, MSUDMV, PP2Ckappa, PP2Cm, PTMP, UG0882E07, protein phosphatase 1K, mitochondrial, PP2C domain-containing protein phosphatase 1K, PP2C-kappa, PP2C-type mitochondrial phosphoprotein phosphatase, branched-chain I+/--ketoacid dehydrogenase phosphatase, branched-chain alpha-ketoacid dehydrogenase phosphatase, protein phosphatase 2C kappa
Ncbi Gene Id Number:
152926
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the PPM family of Mn2+/Mg2+-dependent protein phosphatases. The encoded protein, essential for cell survival and development, is targeted to the mitochondria where it plays a key role in regulation of the mitochondrial permeability transition pore.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-674

List Size:
100 uL

List Price:

$436.00

Out of stock

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