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Western blot analysis of extracts of various cell lines, using PPP1CC antibody (19-252) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts from normal (control) and PPP1CC knockout (KO) HeLa cells, using PPP1CC antibody (19-252) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 1s.
Immunohistochemistry of paraffin-embedded human gastric cancer using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded rat brain using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPP1CC antibody (19-252) at dilution of 1:100 (40x lens).
Western blot analysis of extracts of various cell lines, using PPP1CC antibody (19-252) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.
KO

PPP1CC Antibody, KO Validated

CATALOG NUMBER: 19-252

Clonality:
Polyclonal
Tested Applications:
ChIP, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-323 of human PPP1CC (NP_002701.1).
Conjugate:
Unconjugated
Tested Applications:
ChIP, IHC, WB
Application Note:
WB: 1:200 - 1:2000
IHC: 1:50 - 1:200
CHIP: 1:50 - 1:200
Positive Control 1:
293T
Positive Control 2:
HL-60
Positive Control 3:
Mouse thymus
Positive Control 4:
Mouse spleen
Positive Control 5:
Rat brain
Predicted Molecular Weight:
Observed: 38kDa
Validation:
Antibody is Knockout validated.
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPP1CC
Additional Names:
Serine/threonine-protein phosphatase PP1-gamma catalytic subunit, PP-1G, Protein phosphatase 1C catalytic subunit, PPP1CC
Ncbi Gene Id Number:
5501
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene belongs to the protein phosphatase family, PP1 subfamily. PP1 is an ubiquitous serine/threonine phosphatase that regulates many cellular processes, including cell division. It is expressed in mammalian cells as three closely related isoforms, alpha, beta/delta and gamma, which have distinct localization patterns. This gene encodes the gamma isozyme. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-252

List Size:
100 uL

List Price:

$540.00

Datasheet
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