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Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in HepG2 cell line lysates (35ug/lane).
PPP1R3F Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western Blot at 1:1000 dilution + human heart lysate Lysates/proteins at 20 ug per lane.

PPP1R3F Antibody

CATALOG NUMBER: 57-195

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Mouse
Immunogen:
This PPP1R3F antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 549-578 amino acids from the C-terminal region of human PPP1R3F.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
83 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP1R3F
Additional Names:
Protein phosphatase 1 regulatory subunit 3F, R3F, PPP1R3F
Protein Accession Number:
Q6ZSY5
Protein Gi Number:
226694145
Ncbi Gene Id Number:
89801
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a protein that has been identified asone of several type-1 protein phosphatase (PP1) regulatorysubunits. One or two of these subunits, together with thewell-conserved catalytic subunit, can form the PP1 holoenzyme,where the regulatory subunit functions to regulate substratespecificity and/or targeting to a particular cellular compartment.Alternative splicing results in multiple transcript variants.
Background Reference 1:
Suttner, K., et al. J. Allergy Clin. Immunol. 125(6):1395-1399(2010)
Background Reference 2:
Piton, A., et al. Mol. Psychiatry (2010) In press :
Background Reference 3:
Ceulemans, H., et al. Bioessays 24(4):371-381(2002)
Background Reference 4:
Schindelhauer, D., et al. Genome Res. 6(11):1056-1069(1996)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

57-195

List Size:
400 ul

List Price:

$495.00

Datasheet
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