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45-119 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-119 (0.1ug/ml) staining of Mouse (A), Rat (B) and Pig (C) Brain lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-119 (0.05ug/ml) staining of Human Cerebellum lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PPP2C Antibody

CATALOG NUMBER: 45-119

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Pig, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-EPHVTRRTPDYFL
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 36kDa band observed in Human Brain (Cerebellum), Mouse, Rat and Pig Brain lysates (calculated MW of 35.6kDa according to Human NP_002706.1, Mouse NP_062284.1, Rat NP_058735.1 and Pig NP_999531.1). Recommended concentration: 0.05-0.2ug/ml.
Specificity:
Two variants NP_001009552.1 and NP_004147.1 represent identical protein.
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PPP2CA / PPP2CB
Additional Names:
PPP2CA, protein phosphatase 2 (formerly 2A), catalytic subunit, alpha isoform, PP2Ac, PP2CA, RP-C, PP2A-alpha, protein phosphatase 2, catalytic subunit, alpha isoform, replication protein C, serine/threonine protein phosphatase 2A, catalytic subunit, alpha isoform, serine/threonine-protein phosphatase 2A catalytic subunit alpha isoform, PPP2CB, protein phosphatase 2 (formerly 2A), catalytic subunit, beta isoform, PP2CB, PP2A-beta, protein phosphatase 2, catalytic subunit, beta isoform, protein phosphatase type 2A catalytic subunit, serine/threonine protein phosphatase 2A, catalytic subunit, beta isoform
Protein Accession Number:
NP_002706.1,NP_004147.1
Protein Gi Number:
4506017
Ncbi Gene Id Number:
5515;5516
Background Reference 1:
Hemmings BA, Wernet W, Mayer R, Maurer F, Hofsteenge J, Stone SR. The nucleotide sequence of the cDNA encoding the human lung protein phosphatase 2A beta catalytic subunit. Nucleic Acids Res 1988 Dec 9;16(23):11366

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-119

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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