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45-120 (2ug/ml) staining of Human Thymus (A) and Human Lymph node (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-120 (3.75ug/ml) staining of paraffin embedded Human Thyroid Gland. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-120 (3.75ug/ml) staining of paraffin embedded Human Heart. Steamed antigen retrieval with citrate buffer pH 6, AP-staining. <strong>This data is from a previous batch, not on sale.</strong>
45-120 (2ug/ml) staining of Human Thymus (A) and Human Lymph node (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PPP2R1A Antibody

CATALOG NUMBER: 45-120

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Pig, Cow
Immunogen:
The immunogen for this antibody is: C-KYFAQEALTVLSLA
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:4000.Western Blot:Approx 60-65kDa band observed in Human Thymus and Human Lymph node lysates (calculated MW of 65.3kDa according to NP_055040.2). Recommended concentration: 2-6ug/ml.
Positive Control 1:
Cat. No. 1369 - Human Lymph Node Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PPP2R1A
Additional Names:
PPP2R1A, MGC786, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform, PP2A, subunit A, R1-alpha isoform, PP2A, subunit A, PR65-alpha isoform, medium tumor antigen-associated 61 KDA protein, Serine/threonine protein phosphatase 2A, 65 KDA regulatory subunit A, alpha isoform, protein phosphatase 2 (formerly 2A), regulatory subunit A, alpha isoform, PR65A, alpha isoform of regulatory subunit A, protein phosphatase 2, medium tumor antigen-associated 61 KDA protein, protein phosphatase 2 (formerly 2A), regulatory subunit A (PR 65), alpha isoform
Protein Accession Number:
NP_055040.2
Protein Gi Number:
21361399
Ncbi Gene Id Number:
5518
Background Reference 1:
Hong Y, Sarge KD. Regulation of protein phosphatase 2A activity by heat shock transcription factor 2. J Biol Chem. 1999 May 7;274(19):12967-70.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-120

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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