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Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Immunohistochemical analysis of paraffin-embedded H. kidney section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.
Fluorescent image of Hela cells stained with PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-rabbit lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).
Western blot analysis in ZR-75-1 cell line lysates (35ug/lane).
Immunohistochemical analysis of paraffin-embedded H. liver section using PPP2R2A Antibody (N-term). Antibody was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.

PPP2R2A Antibody

CATALOG NUMBER: 61-002

Clonality:
Polyclonal
Tested Applications:
IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey, Mouse, Pig, Rabbit, Rat
Immunogen:
This PPP2R2A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 43-71 amino acids from the N-terminal region of human PPP2R2A.
Conjugate:
Unconjugated
Tested Applications:
IF, IHC-P, WB
Application Note:
For IHC-P starting dilution is: 1:25
For IF starting dilution is: 1:25
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP2R2A
Additional Names:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B alpha isoform, PP2A subunit B isoform B55-alpha, PP2A subunit B isoform PR55-alpha, PP2A subunit B isoform R2-alpha, PP2A subunit B isoform alpha, PPP2R2A
Protein Accession Number:
P63151
Protein Gi Number:
52783535
Ncbi Gene Id Number:
5520
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The B regulatory subunit might modulate substrate selectivity and catalytic activity, and also might direct the localization of the catalytic enzyme to a particular subcellular compartment.
Background Reference 1:
Mayer R.E., et al. Biochemistry 30:3589-3597(1991).
Background Reference 2:
Ota T., et al. Nat. Genet. 36:40-45(2004).
Background Reference 3:
Nusbaum C., et al. Nature 439:331-335(2006).
Background Reference 4:
Mural R.J., et al. Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-002

List Size:
400 ul

List Price:

$495.00

Datasheet
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