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Western blot analysis of PPP2R2B Antibody in Hela cell line lysates (35ug/lane)
Formalin-fixed and paraffin-embedded human brain tissue with PPP2R2B Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of hela cells using PPP2R2B Antibody (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PPP2R2B Antibody in Hela cell line lysates (35ug/lane)

PPP2R2B Antibody

CATALOG NUMBER: 63-541

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Monkey, Mouse, Pig, Rabbit, Rat, Xenopus
Immunogen:
This PPP2R2B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 104-130 amino acids from the Central region of human PPP2R2B.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
52 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPP2R2B
Additional Names:
Serine/threonine-protein phosphatase 2A 55 kDa regulatory subunit B beta isoform, PP2A subunit B isoform B55-beta, PP2A subunit B isoform PR55-beta, PP2A subunit B isoform R2-beta, PP2A subunit B isoform beta, PPP2R2B
Protein Accession Number:
Q00005
Protein Gi Number:
231446
Ncbi Gene Id Number:
5521
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PPP2R2B belongs to the phosphatase 2 regulatory subunit B family. Protein phosphatase 2 is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. The B regulatory subunit might modulate substrate selectivity and catalytic activity. This protein is a beta isoform of the regulatory subunit B55 subfamily.
Background Reference 1:
Rajkiewicz,M., et.al., Neurol. Neurochir. Pol. 42 (6), 497-504 (2008)
Background Reference 2:
Ben-Israel,H., et.al., J. Virol. 82 (19), 9381-9388 (2008)
Background Reference 3:
Strack,S., et.al., J. Comp. Neurol. 392 (4), 515-527 (1998)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

63-541

List Size:
400 ul

List Price:

$495.00

Datasheet
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