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42-005 (0.5ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-005 (3.75ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-005 (0.5ug/ml) staining of Human Liver lysate (lane 1), Mouse Liver (lane 2) and Rat Liver (lane 3) (35ug protein in RIPA buffer). Detected by chemiluminescence.

PPP2R4 Antibody

CATALOG NUMBER: 42-005

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-GSLLPIHPVTSG
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 37kDa band observed in Human, Mouse and Rat Liver lysates and in lysates of cell line HepG2 (calculated MW of 36.8kDa according to NP_066954.2). Recommended concentration: 0.5-2ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 3.75ug/ml.
Specificity:
This antibody is expected to recognize all reported isoforms (NP_821068.1; NP_066954.2; NP_821070.1). Reported variants NP_066954.2 and NP_821067.1 represent identical protein.
Positive Control 1:
Cat. No. 1304 - Human Liver Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PPP2R4
Additional Names:
PPP2R4, protein phosphatase 2A activator, regulatory subunit 4, MGC2184, PP2A, PR53, PTPA, OTTHUMP00000022333, PP2A phosphatase activator, PP2A, subunit B', phosphotyrosyl phosphatase activator, protein phosphatase 2A, regulatory subunit B', protein phosphatase 2A, regulatory subunit B' (PR 53)
Protein Accession Number:
NP_821068.1,NP_066954.2,NP_821070.1
Protein Gi Number:
30065645
Ncbi Gene Id Number:
5524
Background Reference 1:
Gushiken FC, Patel V, Liu Y, Pradhan S, Bergeron AL, Peng Y, Vijayan KV. Protein phosphatase 2A negatively regulates integrin alpha(IIb)beta(3) signaling. J Biol Chem 2008 May 283 (19): 12862-9.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-005

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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