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46-212 (0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-212 (0.1ug/ml) staining of Human Cerebellum (A), Mouse (B) and Rat (C) Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PPP2R5E Antibody

CATALOG NUMBER: 46-212

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-LKRGLRRDGIIPT
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 50kDa band observed in Human (Cerebellum), Mouse and Rat Brain lysates (calculated MW of 54.7kDa according to Human NP_001269108.1 and Mouse NP_036154.1 and 50.4kDa according to Rat NP_001100210.1). Recommended concentration: 0.1-0.3ug/ml.
Positive Control 1:
Cat. No. 1403 - Mouse Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PPP2R5E
Additional Names:
PPP2R5E, protein phosphatase 2, regulatory subunit B (B56), epsilon isoform, PP2A, B subunit, B' epsilon isoform, PP2A, B subunit, R5 epsilon isoform, PP2A, B subunit, B56 epsilon isoform, PP2A, B subunit, PR61 epsilon isoform, Serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon isoform, protein phosphatase 2, regulatory subunit B', epsilon isoform, PP2A, B subunit, R5 epsilon, epsilon isoform of regulatory subunit B56, protein phosphatase 2A, serine/threonine protein phosphatase 2A, 56 kDa regulatory subunit, epsilon
Protein Accession Number:
NP_006237.1
Protein Gi Number:
5453956
Ncbi Gene Id Number:
5529
Background Reference 1:
McCright B, Virshup DM. Identification of a new family of protein phosphatase 2A regulatory subunits. J Biol Chem 1995 Nov 3;270(44):26123-8

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-212

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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