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Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (23-534) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 5s.
Immunohistochemistry of paraffin-embedded human liver using PPP4R1 antibody (23-534) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using PPP4R1 antibody (23-534) at dilution of 1:100 (40x lens).
Immunoprecipitation analysis of 200ug extracts of 293T cells, using 3 ug PPP4R1 antibody (23-534). Western blot was performed from the immunoprecipitate using PPP4R1 antibody (23-534) at a dilition of 1:1000.
Western blot analysis of extracts of various cell lines, using PPP4R1 antibody (23-534) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 5s.

PPP4R1 Antibody

CATALOG NUMBER: 23-534

Clonality:
Polyclonal
Tested Applications:
IHC, IP, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 324-647 of human PPP4R1 (NP_001035847.1).
Conjugate:
Unconjugated
Tested Applications:
IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:100
IP: 1:50 - 1:100
Positive Control 1:
293T
Positive Control 2:
U-251MG
Positive Control 3:
BT-474
Positive Control 4:
K-562
Positive Control 5:
SKOV3
Predicted Molecular Weight:
Observed: 130kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPP4R1
Additional Names:
MEG1, PP4(Rmeg), PP4R1, serine/threonine-protein phosphatase 4 regulatory subunit 1, serine/threonine phosphatase 4
Ncbi Gene Id Number:
9989
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes one of several alternate regulatory subunits of serine/threonine protein phosphatase 4 (PP4). The protein features multiple HEAT repeats. This protein forms a complex with PP4RC. This complex may have a distinct role from other PP4 complexes, including regulation of HDAC3 (Zhang et al., PMID: 15805470). There is also a transcribed pseudogene on chromosome 20. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

23-534

List Size:
100 uL

List Price:

$436.00

Datasheet
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