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Western blot analysis of extracts of various cell lines, using PPP6C antibody (19-253) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Enhanced Kit.<br/>Exposure time: 90s.
Western blot analysis of extracts of various cell lines, using PPP6C antibody (19-253) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit.
Exposure time: 90s.

PPP6C Antibody

CATALOG NUMBER: 19-253

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-305 of human PPP6C (NP_002712.1).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
293T
Positive Control 2:
SH-SY5Y
Positive Control 3:
HepG2
Positive Control 4:
SW480
Positive Control 5:
Mouse kidney
Positive Control 6:
Mouse thymus
Predicted Molecular Weight:
Observed: 35kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PPP6C
Additional Names:
PPP6C, PP6, PP6C, PPP6, Protein phosphatase 6
Ncbi Gene Id Number:
5537
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the catalytic subunit of protein phosphatase, a component of a signaling pathway regulating cell cycle progression. Splice variants encoding different protein isoforms exist. The pseudogene of this gene is located on chromosome X.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-253

List Size:
100 uL

List Price:

$436.00

Out of stock

Datasheet
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