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Western blot analysis in A549 cell line lysates (35ug/lane).
Western blot analysis of PPT1 using rabbit polyclonal PPT1 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PPT1 gene.
Western blot analysis in A549 cell line lysates (35ug/lane).

PPT1 Antibody

CATALOG NUMBER: 58-985

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PPT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 189-217 amino acids from the Central region of human PPT1.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
34 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PPT1
Additional Names:
Palmitoyl-protein thioesterase 1, PPT-1, Palmitoyl-protein hydrolase 1, PPT1, PPT
Protein Accession Number:
P50897
Protein Gi Number:
1709747
Ncbi Gene Id Number:
5538
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a small glycoproteininvolved in the catabolism of lipid-modified proteins duringlysosomal degradation. The encoded enzyme removes thioester-linkedfatty acyl groups such as palmitate from cysteine residues. Defectsin this gene are a cause of infantile neuronal ceroidlipofuscinosis 1 (CLN1, or INCL) and neuronal ceroid lipofuscinosis4 (CLN4). Two transcript variants encoding different isoforms havebeen found for this gene.
Background Reference 1:
Ohno, K., et al. Brain Dev. 32(7):524-530(2010)
Background Reference 2:
Simonati, A., et al. Pediatr. Neurol. 40(4):271-276(2009)
Background Reference 3:
Martins-de-Souza, D., et al. Eur Arch Psychiatry Clin Neurosci 259(3):151-163(2009)
Background Reference 4:
Kousi, M., et al. Brain 132 (PT 3), 810-819 (2009) :

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-985

List Size:
400 ul

List Price:

$495.00

Datasheet
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