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Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis of MAPKAPK5 using rabbit polyclonal MAPKAPK5 Antibody (T182) using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MAPKAPK5 gene.
Western Blot at 1:1000 dilution + Hela whole cell lysate Lysates/proteins at 20 ug per lane.

PRAK Antibody

CATALOG NUMBER: 62-736

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PRAK(MAPKAPK5) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 160-189 amino acids from human PRAK(MAPKAPK5).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPKAPK5
Additional Names:
MAP kinase-activated protein kinase 5, MAPK-activated protein kinase 5, MAPKAP kinase 5, MAPKAP-K5, MAPKAPK-5, MK-5, MK5, p38-regulated/activated protein kinase, PRAK, MAPKAPK5, PRAK
Protein Accession Number:
Q8IW41
Protein Gi Number:
52000829
Ncbi Gene Id Number:
8550
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
MAPKAPK5 is a member of the serine/threonine kinase family. In response to cellular stress and proinflammatory cytokines, this kinase is activated through its phosphorylation by MAP kinases including MAPK1/ERK, MAPK14/p38-alpha, and MAPK11/p38-beta. In vitro, this kinase phosphorylates heat shock protein HSP27 at its physiologically relevant sites.
Background Reference 1:
New, L., et al., EMBO J. 17(12):3372-3384 (1998).
Background Reference 2:
Ni, H., et al., Biochem. Biophys. Res. Commun. 243(2):492-496 (1998).
Background Reference 3:
Sudo T.,Biochem. Biophys. Res. Commun. 269:521-525(2000).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

62-736

List Size:
400 ul

List Price:

$495.00

Datasheet
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