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Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.
Immunohistochemical analysis of paraffin - embedded human normal spleen (top) and human breast carcinoma (bottom) showing nuclear / cytoplasma location using PRAK antibody with DAB staining.
Western blot analysis using PRAK monoclonal antibody against truncated PRAK - His recombinant protein.

PRAK Antibody [7H10B4]

CATALOG NUMBER: 32-205

Clonality:
Monoclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Mouse
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human
Immunogen:
Ni-NTA purified truncated recombinant PRAK-His expressed in E. Coli strain BL21 (DE3).
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Western Blot:1:500 - 1:2,000
IHC(P):1:500 - 1:2,000
ELISA:Propose dilution 1:10,000.
Determining optimal working dilutions by titration test.
Clonality:
Monoclonal
Isotype:
IgG1
Buffer:
Ascitic fluid containing 0.03% sodium azide.
Concentration:
batch dependent
Storage Conditions:
PRAK monoclonal antibody can be stored at -20°C, stable for one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
MAPKAPK5
Additional Names:
PRAKMAPK-activated protein kinase 5, MK5, MK-5, PRAK, MAPKAP-K5
Protein Accession Number:
Q8IW41
Protein Gi Number:
52000829
Ncbi Gene Id Number:
8550
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRAK (p38-regulated /activated kinase), also referred to as mitogen-activated protein kinase (MAPK)-activated protein kinase (MAPKAPK)-5, is an ubiquitously expressed serine/threonine kinase regulated by p38α and p38β MAP kinases. Activated JNK, p38γ or p38δ are unable to induce phosphorylation of PRAK in vitro. Phosphorylation of PRAK occurs in vivo in response to p38 activation by stress-related extracellular stimuli including UV light, oxidation and proinflammatory cytokines. Two other substrates for p38, MAPKAPK-2 and MAPKAPK-3/3pK, share approximately 45% sequence homology with PRAK including the phosphorylation motif recognized by p38, Lys-X-Thr-Pro. Activated PRAK has been shown to specifically phosphorylate HSP 27 in vitro, suggesting that the protein may play a role in stress-induced small heat shock protein phosphorylation in vivo.
Background Reference 1:
Paliga AJ. Natale DR. Watson AJ. Biol Cell. 2005, Aug, 97(8):629-40.
Background Reference 2:
Wijtten PJ. Prak R. Lemme A. et al. Br Poult Sci. 2004, Aug, 45(4):504-11.
Background Reference 3:
New L. Jiang Y. Han J. Mol Biol Cell. 2003, Jun, 14(6):2603-16. Epub 2003 Mar 20.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

32-205

List Size:
0.1 mL

List Price:

$432.00

Datasheet
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