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Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Immunohistochemistry of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 2.5 μg/mL.
Immunofluorescence of PRC1 in mouse skeletal muscle tissue with PRC1 antibody at 20 μg/mL.
Western blot analysis of PRC1 in human skeletal muscle tissue lysate with Prc1 antibody at (A) 0.5 μg/ml and (B) 1 μg/mL.
Trial Size

PRC1 Antibody

CATALOG NUMBER: 6821

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PRC1 antibody was raised against a 19 amino acid synthetic peptide near the center of human PRC1.
The immunogen is located within amino acids 350 - 400 of PRC1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, IHC-P, WB
Application Note:
PRC1 antibody can be used for detection of Prc1 by Western blot at 0.5 - 1 μg/mL. Antibody can also be used for immunohistochemistry starting at 2.5 μg/mL. For immunofluorescence start at 2.5 μg/mL.
Antibody validated: Western Blot in human samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
Specificity:
At least three alternatively spliced transcript variants encoding distinct isoforms have been observed.
Positive Control 1:
Cat. No. 1375 - Human Skeletal Muscle Tissue Lysate
Purification:
PRC1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRC1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRC1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRC1
Additional Names:
PRC1 Antibody: ASE1, Protein regulator of cytokinesis 1
Protein Accession Number:
NP_003972
Protein Gi Number:
4506039
Ncbi Gene Id Number:
9055
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRC1 Antibody: PRC1 (Protein Regulator of Cytokinesis 1) is a key regulator of cytokinesis and has been identified as a substrate for several cyclin-dependent kinases (CDKs). High levels of PRC1 expression occurs during S and G2/M and drops dramatically after mitosis exit and G1 entry. It is located in the nucleus during interphase and binds to the midzone of mitotic spindles during anaphase and is localized to the cell midbody during cytokinesis. Depletion of PRC1 has been shown to prevent cellular cleavage, but it has no effect on nuclear division, demonstrating the importance of PRC1 in mitosis.
Background Reference 1:
Jiang W, Jimenez G, Wells NJ, et al. PRC1: a human mitotic spindle-associated Cdk substrate protein required for cytokinesis. Mol. Cell 1998; 2:877-85.
Background Reference 2:
Pellman D, Bagget M, Tu H, et al. Two microtubule-associated proteins required for anaphase spindle movement in Saccharomyces Cerevisiae. J. Cell Biol. 1995; 130:1373-85.
Background Reference 3:
Kurasawa Y, Earnshaw WC, Mochizuki Y, et al. Essential roles of KIF4 and its binding partner PRC1 in organized central spindle midzone formation. EMBO J. 2004; 23:3237-48.
Background Reference 4:
Mollinari C, Kleman JP, Jiang W, et al. PRC1 is a microtubule binding and bundling protein essential to maintain the mitotic spindle midzone. J. Cell Biol. 2002; 157:1175-86.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6821

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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