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46-216 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f
46-216 Flow cytometric analysis of paraformaldehyde fixed HEK293 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) f

PRDM16 Antibody

CATALOG NUMBER: 46-216

Clonality:
Polyclonal
Tested Applications:
ChIP, ELISA, Flow
Host Species:
Goat
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse
Immunogen:
The immunogen for this antibody is: C-TSESGAFHPINHL
Conjugate:
Unconjugated
Tested Applications:
ChIP, ELISA, Flow
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Flow Cytometry: Flow cytometric analysis of HEK293 cells. Recommended concentration: 10ug/ml.

ChIP: This product has been successfully used in ChIP on Mouse: Hondares E et al. (2011) Cell Transplant. 2011;20(8):1179-92. PMID: 21294954.

Specificity:
This antibody is expected to recognise both reported isoforms.
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PRDM16
Additional Names:
PRDM16, MEL1, KIAA1675, PR domain containing 16, transcription factor MEL1, MGC166915, PFM13, MDS1/EVI1-like, PR-domain zinc finger protein 16
Protein Accession Number:
NP_071397.3,NP_955533.2
Protein Gi Number:
289547573
Ncbi Gene Id Number:
63976
Background Reference 1:
Mochizuki N, Shimizu S, Nagasawa T, Tanaka H, Taniwaki M, Yokota J, Morishita K. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood 2000 Nov 1;96(9):3209-14

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-216

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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