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<strong> Figure 1 WB Validation in MCF7 Cells </strong><br>Loading: 15 μg of lysate Antibodies: PRDM16, 5555, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 2 WB Validation in Mouse Lung </strong><br>Loading: 15 μg of lysate Antibodies: PRDM16, 5555, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 3 WB Validation in Rat Spleen </strong><br>Loading: 15 μg of lysate Antibodies: PRDM16, 5555, 2 μg/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
<strong>Figure 4 Immunohistochemistry Validation of PRDM16 in Human Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PRDM16 antibody (5555) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 5 Immunohistochemistry Validation of PRDM16 in Human Brain</strong><br> Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-PRDM16 antibody (5555) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 6 Immunohistochemistry Validation of PRDM16 in Mouse Kidney</strong><br> Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PRDM16 antibody (5555) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 7 Immunohistochemistry Validation of PRDM16 in Rat Spleen</strong><br> Immunohistochemical analysis of paraffin-embedded rat spleen tissue using anti-PRDM16 antibody (5555) at 2 μg/ml. Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4°C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin.
<strong>Figure 8 Immunofluorescence Validation of PRDM16 in Mouse Brain</strong><br> Immunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain tissue labeling PRDM16 with 5555 at 20 μg/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).
Figure 1 WB Validation in MCF7 Cells
Loading: 15 μg of lysate Antibodies: PRDM16, 5555, 1 μ g/mL , 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10000 dilution.
Trial Size

PRDM16 Antibody

CATALOG NUMBER: 5555

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Anti-PRDM16 antibody (5555) was raised against a peptide corresponding to 17 amino acid synthetic peptide near the carboxy terminus of human PRDM16.

The immunogen is located within amino acids 1120-1170 of PRDM16.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC-P, WB, IHC-P/IF
Application Note:
WB: 1-4 μg/mL; IHC-P: 1-2 μg/mL; IHC-P/IF: 10-20 μg/mL.

Antibody validated: Western Blot in human, mouse and rat samples; Immunohistochemistry in human, mouse, and rat samples; Immunofluorescence in the mouse sample. All other applications and species not yet tested.
Positive Control 1:
Cat. No. 1402 - Mouse Lung Tissue Lysate
Positive Control 2:
Cat. No. 1466 - Rat Spleen Tissue Lysate
Predicted Molecular Weight:
Predicted: 140kD, 138kD, 120 kD

Observed: 130 kD
Purification:
PRDM16 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRDM16 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRDM16 antibody can be stored at 4°C up to one year. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRDM16
Additional Names:
PRDM16 Antibody: MEL1, LVNC8, PFM13, CMD1LL, KIAA1675, MEL1, PR domain zinc finger protein 16, PR domain-containing protein 16
Protein Accession Number:
CAH71530
Protein Gi Number:
55665817
Ncbi Gene Id Number:
63976
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRDM16 Antibody: PRDM16 is a zinc finger transcription factor and contains an N-terminal PR domain. The reciprocal translocation t(1;3)(p36;q21) occurs in a subset of myelodysplastic syndrome (MDS) and acute myeloid leukemia (AML). This gene is located near the 1p36.3 breakpoint and has been shown to be specifically expressed in the t(1:3)(p36, q21)-positive MDS/AML. The translocation results in the overexpression of a truncated version of this protein that lacks the PR domain, which may play an important role in the pathogenesis of MDS and AML. Recent studies have shown that PRDM16 normally acts as a Smad3 binding protein that may be important for the development of orofacial structures through modulation of the TGF-beta signaling pathway. Other experiments have indicated that PRDM16 controls a bidirectional cell fate switch between skeletal myoblasts and brown fat cells.
Background Reference 1:
Mochizuki N, Shimizu S, Nagasawa T, et al. A novel gene, MEL1, mapped to 1p36.3 is highly homologous to the MDS1/EVI1 gene and is transcriptionally activated in t(1;3)(p36;q21)-positive leukemia cells. Blood2000; 96:3209-14.
Background Reference 2:
Morishita K. Leukemogenesis of the EVI1/MEL1 gene family. Int. J. Hematol.2007; 85:279-86.
Background Reference 3:
Warner DR, Horn KH, Mudd L, et al. PRDM16/MEL1: a novel Smad binding protein expressed in murine embryonic orofacial tissue. Biochim. Biophys. Acta2007; 1773:814-20.
Background Reference 4:
Seale P, Bjork B, Yang W, et al. PRDM16 controls a brown fat/skeletal muscle switch. Nature2008; 454:961-7.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

5555

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
Shipping Info

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$35 Dry Ice Shipment fee may be required on some items.

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