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Western blot analysis of extracts of various cell lines, using PRDM5 antibody (22-890) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse liver using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse spleen using PRDM5 antibody (22-890) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of A549 cells using PRDM5 antibody (22-890). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PRDM5 antibody (22-890) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 90s.

PRDM5 Antibody

CATALOG NUMBER: 22-890

Clonality:
Polyclonal
Tested Applications:
IF, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human PRDM5 (NP_061169.2).
Conjugate:
Unconjugated
Tested Applications:
IF, IHC, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
IF: 1:50 - 1:200
Positive Control 1:
Mouse kidney
Positive Control 2:
Mouse heart
Positive Control 3:
Mouse lung
Positive Control 4:
Mouse ovary
Positive Control 5:
Rat kidney
Positive Control 6:
Rat liver
Predicted Molecular Weight:
Observed: 110kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRDM5
Additional Names:
PRDM5, PFM2, BCS2
Ncbi Gene Id Number:
11107
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a transcription factor of the PR-domain protein family. It contains a PR-domain and multiple zinc finger motifs. Transcription factors of the PR-domain family are known to be involved in cell differentiation and tumorigenesis.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

22-890

List Size:
100 uL

List Price:

$436.00

Out of stock

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