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Prdm9 Antibody

CATALOG NUMBER: 43-368

Clonality:
Polyclonal
Tested Applications:
ELISA
Host Species:
Goat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Homology:
Expected Species Reactivity based on sequence homology: Mouse
Immunogen:
The immunogen for this antibody is: C-QEQHVDSQNKNDK
Conjugate:
Unconjugated
Tested Applications:
ELISA
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Preliminary experiments gave an approx 70kDa band in Mouse fetal Brain lysates and in lysates of cell line NIH3T3 after 0.1ug/ml antibody staining. Please note that currently we cannot find an explanation in the literature for the band we observe given the calculated size of 97.8kDa according to NP_659058.2. The 70kDa band was successfully blocked by incubation with the immunizing peptide. We would appreciate any feedback from people in the field - have any results been reported with other antibodies/lysates? Have any further splice variants/modified forms been reported?
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
Prdm9
Additional Names:
Prdm9, PR domain containing 9, RP23-64O5.3, BC012016, Dsbc1, G1-419-29, Meisetz, Rcr1, repro7, PR domain zinc finger protein 9, PR domain-containing protein 9, PR-domain zinc finger protein, VPR domain containing 9, histone-lysine N-methyltransferase PRDM9, hybrid sterility protein 1, meiosis-induced factor containing a PR/SET domain and zinc-finger motif
Protein Accession Number:
NP_659058.2
Protein Gi Number:
226958682
Ncbi Gene Id Number:
213389
Background Reference 1:
Borel C, Cheung F, Stewart H, Koolen DA, Phillips C, Thomas NS, Jacobs PA, Eliez S, Sharp AJ.Evaluation of PRDM9 variation as a risk factor for recurrent genomic disorders and chromosomal non-disjunction.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-368

List Size:
0.1 mg

List Price:

$391.00

Datasheet
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