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Western blot analysis of extracts of various cell lines, using PRDX1 antibody (15-042) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Immunofluorescence analysis of HeLa cells using PRDX1 antibody (15-042). Blue: DAPI for nuclear staining.
Western blot analysis of extracts of various cell lines, using PRDX1 antibody (15-042) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PRDX1 Antibody

CATALOG NUMBER: 15-042

Clonality:
Polyclonal
Tested Applications:
IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1-199 of human PRDX1 (NP_001189360.1).
Conjugate:
Unconjugated
Tested Applications:
IF, WB
Application Note:
WB: 1:500 - 1:2000
IF: 1:50 - 1:200
Positive Control 1:
HepG2
Positive Control 2:
HeLa
Positive Control 3:
A-549
Positive Control 4:
Mouse liver
Positive Control 5:
Mouse heart
Predicted Molecular Weight:
Observed: 24kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRDX1
Additional Names:
PRDX1, peroxiredoxin 1, MSP23, NKEFA, PAG, PAGA, PAGB, PRX1, PRXI, TDPX2, natural killer-enhancing factor A, proliferation-associated gene A, thioredoxin-dependent peroxide reductase 2
Ncbi Gene Id Number:
5052
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. The encoded protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. Four transcript variants encoding the same protein have been identified for this gene.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

15-042

List Size:
100 uL

List Price:

$436.00

Datasheet
Shipping Info

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