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Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.
Western blot analysis in Hela cell line lysates (35ug/lane).
Formalin-fixed and paraffin-embedded human brain tissue reacted with PRDX2 Antibody , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western Blot at 1:1000 dilution + Raji whole cell lysate Lysates/proteins at 20 ug per lane.

PRDX2 Antibody

CATALOG NUMBER: 61-603

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Monkey
Immunogen:
This PRDX2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 98-127 amino acids from the Central region of human PRDX2.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
22 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRDX2
Additional Names:
Peroxiredoxin-2, Natural killer cell-enhancing factor B, NKEF-B, PRP, Thiol-specific antioxidant protein, TSA, Thioredoxin peroxidase 1, Thioredoxin-dependent peroxide reductase 1, PRDX2, NKEFB, TDPX1
Protein Accession Number:
P32119
Protein Gi Number:
2507169
Ncbi Gene Id Number:
7001
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRDX2 is a member of the peroxiredoxin family of antioxidant enzymes, which reduce hydrogen peroxide and alkyl hydroperoxides. This protein may play an antioxidant protective role in cells, and may contribute to the antiviral activity of CD8(+) T-cells. This protein may have a proliferative effect and play a role in cancer development or progression. The crystal structure of this protein has been resolved to 2.7 angstroms.
Background Reference 1:
Engstrom,K.S., et.al., Mutat. Res. (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-603

List Size:
400 ul

List Price:

$495.00

Datasheet
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