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Western blot analysis of PRDX3 Antibody in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane)(2ug/ml)
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with PRDX3 Antibody (N-term), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Flow cytometric analysis of MDA-MB468 cells using PRDX3 Antibody (N-term)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
Western blot analysis of PRDX3 using rabbit polyclonal PRDX3 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX3 gene.
Western blot analysis of PRDX3 Antibody in NCI-H460,MDA-MB231,HepG2 cell line lysates (35ug/lane)(2ug/ml)

PRDX3 Antibody

CATALOG NUMBER: 61-604

Clonality:
Polyclonal
Tested Applications:
Flow, IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PRDX3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 66-94 amino acids from the N-terminal region of human PRDX3.
Conjugate:
Unconjugated
Tested Applications:
Flow, IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
For FACS starting dilution is: 1:10~50
Predicted Molecular Weight:
28 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRDX3
Additional Names:
Thioredoxin-dependent peroxide reductase, mitochondrial, Antioxidant protein 1, AOP-1, HBC189, Peroxiredoxin III, Prx-III, Peroxiredoxin-3, Protein MER5 homolog, PRDX3, AOP1
Protein Accession Number:
P30048
Protein Gi Number:
2507171
Ncbi Gene Id Number:
10935
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRDX3 is a protein with antioxidant function and is localized in the mitochondrion. This protein is involved in redox regulation of the cell and Protects radical-sensitive enzymes from oxidative damage by a radical-generating system.It Acts synergistically with MAP3K13 to regulate the activation of NF-kappa-B in the cytosol.
Background Reference 1:
Cox,A.G., et.al., Biochemistry 48 (27), 6495-6501 (2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

61-604

List Size:
400 ul

List Price:

$495.00

Datasheet
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