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Western blot analysis in NCI-H292 cell line lysates (35ug/lane).
Western blot analysis of PRDX4 using rabbit polyclonal PRDX4 Antibody using 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the PRDX4 gene.
Western blot analysis in mouse NIH-3T3 cell line lysates (35ug/lane).
PRDX4 Antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human pancreas tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.
Western blot analysis in NCI-H292 cell line lysates (35ug/lane).

PRDX4 Antibody

CATALOG NUMBER: 56-229

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Rat
Immunogen:
This PRDX4 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 82-110 amino acids from the Central region of human PRDX4.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:50~100
Predicted Molecular Weight:
31 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRDX4
Additional Names:
Peroxiredoxin-4, Antioxidant enzyme AOE372, AOE37-2, Peroxiredoxin IV, Prx-IV, Thioredoxin peroxidase AO372, Thioredoxin-dependent peroxide reductase A0372, PRDX4
Protein Accession Number:
Q13162
Protein Gi Number:
3024727
Ncbi Gene Id Number:
10549
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is an antioxidant enzymeand belongs to the peroxiredoxin family. The protein is localizedto the cytoplasm. Peroxidases of the peroxiredoxin family reducehydrogen peroxide and alkyl hydroperoxides to water and alcoholwith the use of reducing equivalents derived from thiol-containingdonor molecules. This protein has been found to play a regulatoryrole in the activation of the transcription factor NF-kappaB.
Background Reference 1:
Jamaluddin, M., et al. J. Virol. 84(18):9533-9545(2010)
Background Reference 2:
Davila, S., et al. Genes Immun. 11(3):232-238(2010)
Background Reference 3:
Edvardsen, H., et al. Pharmacogenomics J. (2010) In press :
Background Reference 4:
Wang, H.Q., et al. FEBS Lett. 583(9):1511-1515(2009)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

56-229

List Size:
400 ul

List Price:

$495.00

Datasheet
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