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42-175 (1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
42-175 (10ug/ml) staining of paraffin embedded Human Testis. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-175 (1ug/ml) staining of Human Temporal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PREX1 Antibody

CATALOG NUMBER: 42-175

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog, Cow
Immunogen:
The immunogen for this antibody is: C-ERSNLPTDASTTA
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:16000.Western Blot:Approx 150kDa band observed in Human Brain (Temporal Cortex) lysates (calculated MW of 186kDa according to NP_065871.2). Recommended concentration: 1-3ug/ml.Immunohistochemistry:Paraffin embedded Human Testis. Recommended concentration: 10ug/ml.
Positive Control 1:
Cat. No. 1378 - Human Temporal Lobe Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PREX1
Additional Names:
PREX1, phosphatidylinositol-3,4,5-trisphosphate-dependent Rac exchange factor 1, KIAA1415, P-REX1, OTTHUMP00000031228, phosphatidylinositol 3,4,5-trisphosphate-dependent Rac exchanger 1
Protein Accession Number:
NP_065871.2
Protein Gi Number:
34452732
Ncbi Gene Id Number:
57580
Background Reference 1:
Qin J, Xie Y, Wang B, Hoshino M, Wolff DW, Zhao J, Scofield MA, Dowd FJ, Lin MF, Tu Y. Upregulation of PIP3-dependent Rac exchanger 1 (P-Rex1) promotes prostate cancer metastasis. Oncogene 2009 Apr 28 (16): 1853-63.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-175

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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