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Western blot analysis of extracts of LO2 cells, using PRG4 antibody (16-021) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 10s.
Western blot analysis of extracts of LO2 cells, using PRG4 antibody (16-021) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 10s.

PRG4 Antibody

CATALOG NUMBER: 16-021

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Rat
Immunogen:
Recombinant fusion protein containing a sequence corresponding to amino acids 1264-1363 of human PRG4 (NP_001121180.2).
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:200 - 1:2000
Positive Control 1:
LO2
Predicted Molecular Weight:
Observed: 150kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRG4
Additional Names:
PRG4, proteoglycan 4, CACP, FLJ32635, HAPO, JCAP, MSF, SZP, bG174L6.2, Jacobs camptodactyly-arthropathy-pericarditis syndrome, articular superficial zone protein, bG174L6.2 (MSF: megakaryocyte stimulating factor ), camptodactyly, arthropathy, coxa vara,
Ncbi Gene Id Number:
10216
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a large proteoglycan that is synthesized by chondrocytes located at the surface of articular cartilage and by some synovial lining cells. This protein contains both chondroitin sulfate and keratan sulfate glycosaminoglycans. It functions as a boundary lubricant at the cartilage surface and contributes to the elastic absorption and energy dissipation of synovial fluid. Mutations in this gene result in camptodactyly-arthropathy-coxa vara-pericarditis syndrome. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

16-021

List Size:
100 uL

List Price:

$436.00

Datasheet
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