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Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.
Immunofluorescence of PRICKLE1 in human bladder cells with PRICKLE1 antibody at 20 μg/mL.
Western blot analysis of PRICKLE1 in human bladder tissue lysate with PRICKLE1 antibody at 1 μg/mL.
Trial Size

PRICKLE1 Antibody

CATALOG NUMBER: 6561

Clonality:
Polyclonal
Tested Applications:
ELISA, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
PRICKLE1 antibody was raised against a 19 amino acid synthetic peptide near the carboxy terminus of human PRICKLE1.
The immunogen is located within the last 50 amino acids of PRICKLE1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IF, WB
Application Note:
PRICKLE1 antibody can be used for detection of PRICKLE1 by Western blot at 1 μg/mL. Antibody can also be used for immunofluorescence starting at 20 μg/mL. For immunofluorescence start at 20 μg/mL.
Antibody validated: Western Blot in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Multiple isoforms of PRICKLE1 are known to exist. PRICKLE1 antibody is predicted to not cross-react with other PRICKLE protein family members.
Positive Control 1:
Cat. No. 1310 - Human Bladder Tissue Lysate
Predicted Molecular Weight:
Predicted: 46, 91 kDa
Observed: 52, 90 kDa
Purification:
PRICKLE1 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRICKLE1 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRICKLE1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRICKLE1
Additional Names:
PRICKLE1 Antibody: RILP, EPM1B, RILP, Prickle-like protein 1
Protein Accession Number:
NP_694571
Protein Gi Number:
222136684
Ncbi Gene Id Number:
144165
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRICKLE1 Antibody: PRICKLE1, also known as RILP or EPM1B, is a Disheveled-associated protein that serves as a nuclear translocation receptor for REST/NRSF and REST4 (1, 2). It contains three N-terminal LIM domains and three C-terminal nuclear localization signals. It localizes to the cytoplasm, as well as to the nuclear membrane and expressed at higher levels in placenta. PRICKLE1 is a negative regulator of the Wnt / beta-catenin signaling pathway and is a putative tumor suppressor in human HCCs. Defects in the gene encoding PRICKLE1 are associated with autosomal recessive progressive myoclonic epilepsy.
Background Reference 1:
Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56
Background Reference 2:
Shimojo M and Hersh LB. REST/NRSF-interacting LIM domain protein, a putative nuclear translocation receptor. Mol. Cell. Biol. 2003; 23:9025-31
Background Reference 3:
Chan DW, Chan CY, Yam JW, et al. Prickle1 negatively regulates Wnt/β-catenin pathway by promoting Disheveled ubiquitination/degradation in liver cancer. Gastroenterology 2006; 131:1218-27.
Background Reference 4:
Tao H, Manak JR, Sowers L, et al. Mutations in prickle orthologs cause seizures in flies, mice, and humans. Am. J. Hum. Genet. 2011; 88:138-49.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6561

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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