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Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.
Immunocytochemistry of PRICKLE4 in A549 cells with PRICKLE4 antibody at 5 μg/mL.
Immunofluorescence of PRICKL4 in A549 cells with PRICKLE4 antibody at 20 μg/mL.
Western blot analysis of PRICKLE4 in A549 cell lysate with PRICKLE4 antibody at 0.25 μg/ml in the (A) absence and (B) presence of blocking peptide.
Trial Size

PRICKLE4 Antibody

CATALOG NUMBER: 6567

Clonality:
Polyclonal
Tested Applications:
ELISA, ICC, IF, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
PRICKLE4 antibody was raised against an 18 amino acid synthetic peptide near the amino terminus of human PRICKLE4.
The immunogen is located within the first 50 amino acids of PRICKLE4.
Conjugate:
Unconjugated
Tested Applications:
ELISA, ICC, IF, WB
Application Note:
PRICKLE4 antibody can be used for detection of PRICKLE4 by Western blot at 0.25 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL. For immunofluorescence start at 5 μg/mL.
Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
Specificity:
Two isoforms of PRICKLE4 are known to exist. PRICKLE4 antibody is predicted to not cross-react with other PRICKLE protein family members.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Positive Control 2:
Cat. No. 17-003 - A549 Cell Slide
Purification:
PRICKLE4 Antibody is affinity chromatography purified via peptide column.
Clonality:
Polyclonal
Isotype:
IgG
physical-state:
Liquid
Buffer:
PRICKLE4 Antibody is supplied in PBS containing 0.02% sodium azide.
Concentration:
1 mg/mL
Storage Conditions:
PRICKLE4 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRICKLE4
Additional Names:
PRICKLE4 Antibody: OBTP, OEBT, TOMM6, C6orf49, Prickle-like protein 4, Overexpressed breast tumor protein
Protein Accession Number:
NP_037529
Protein Gi Number:
118722347
Ncbi Gene Id Number:
29964
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRICKLE4 Antibody: PRICKLE4, also known as over-expressed breast tumor protein (OBTP), is a member of the LIM family of proteins. It possesses two LIM domains as well as a PET domain and is thought to localize to the nucleus. Its expression in multiple malignant tissue differentiations indicate that it may play a role in cancer differentiation. Two other members of the LIM family, PRICKLE1 and PRICKLE2, are thought to function in the noncanonical WNT signaling pathway which regulates intracellular calcium release and planar cell polarity, suggesting the PRICKLE4 may play a similar role.
Background Reference 1:
Teufel A, Weinmann A, Galle PR, et al. Characterization of OEBT, a LIM protein. Int. J. Mol. Med. 2005; 15:513-8.
Background Reference 2:
Katoh M and Katoh M. Identification and characterization of human PRICKLE1 and PRICKLE2 genes as well as mouse Prickle1 and Prickle2 genes homologous to Drosophila tissue polarity gene prickle. Int. J. Mol. Med. 2003; 11:249-56
Background Reference 3:
Veeman MT, Slusarski DC, Kaykas A, et al. Zebrafish prickle, a modulator of noncanonical Wnt/Fz signaling, regulates gastrulation movements. Curr. Biol. 2003; 13:680-5

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

6567

List Size:
0.02 mg, 0.1 mg

List Price:

Price range: $99.00 through $445.00

Datasheet
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