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Antibody used in WB on Human K562 at 1.25 ug/ml.
Antibody used in WB on Human K562 at 1.25 ug/ml.

PRIM1 Antibody

CATALOG NUMBER: 30-134

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRIM1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PRIM1 antibody can be used for detection of PRIM1 by ELISA at 1:1562500. PRIM1 antibody can be used for detection of PRIM1 by western blot at 1.25 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1204 - K562 Cell Lysate
Predicted Molecular Weight:
50 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PRIM1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PRIM1
Additional Names:
PRIM1, MGC12308, p49
Protein Accession Number:
NP_000937
Protein Gi Number:
4506051
Ncbi Gene Id Number:
5557
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. PRIM1 is the small, 49 kDa primase subunit.The replication of DNA in eukaryotic cells is carried out by a complex chromosomal replication apparatus, in which DNA polymerase alpha and primase are two key enzymatic components. Primase, which is a heterodimer of a small subunit and a large subunit, synthesizes small RNA primers for the Okazaki fragments made during discontinuous DNA replication. The protein encoded by this gene is the small, 49 kDa primase subunit.
Background Reference 1:
Arezi, B., (1999) Biochemistry 38 (39), 12899-12907.

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-134

List Size:
100 ul

List Price:

$473.00

Datasheet
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