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Western blot analysis in NCI-H460 cell line lysates (35ug/lane).
Western blot analysis in NCI-H460 cell line lysates (35ug/lane).

PRIM2 Antibody

CATALOG NUMBER: 59-198

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PRIM2 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-345 amino acids from the Central region of human PRIM2.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
59 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRIM2
Additional Names:
DNA primase large subunit, 277-, DNA primase 58 kDa subunit, p58, PRIM2, PRIM2A
Protein Accession Number:
P49643
Protein Gi Number:
51338777
Ncbi Gene Id Number:
5558
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The replication of DNA in eukaryotic cells is carried outby a complex chromosomal replication apparatus, in which DNApolymerase alpha and primase are two key enzymatic components.Primase, which is a heterodimer of a small subunit and a largesubunit, synthesizes small RNA primers for the Okazaki fragmentsmade during discontinuous DNA replication. The protein encoded bythis gene is the large, 58 kDa primase subunit. [provided byRefSeq].
Background Reference 1:
Vaithiyalingam, S., et al. Proc. Natl. Acad. Sci. U.S.A. 107(31):13684-13689(2010)
Background Reference 2:
Rose, J.E., et al. Mol. Med. 16 (7-8), 247-253 (2010) :
Background Reference 3:
Weiner, B.E., et al. J. Biol. Chem. 282(46):33444-33451(2007)
Background Reference 4:
Rush, J., et al. Nat. Biotechnol. 23(1):94-101(2005)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-198

List Size:
400 ul

List Price:

$495.00

Datasheet
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