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45-123 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
45-123 (0.3ug/ml) staining of Human Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Prion Protein Antibody

CATALOG NUMBER: 45-123

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Pig, Cow
Immunogen:
The immunogen for this antibody is: C-SDYEDRYYREN
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 25kDa band observed in Human Brain lysates (calculated MW of 27.7kDa according to NP_000302.1 and NP_898902.1). Recommended concentration: 0.3-1ug/ml.
Specificity:
Reported variants represent identical protein (NP_000302.1; NP_898902.1; NP_001073590.1; NP_001073591.1; NP_001073592.1).
Positive Control 1:
Cat. No. 1303 - Human Brain Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PRNP
Additional Names:
PRNP , prion protein (p27-30) (Creutzfeldt-Jakob disease, Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia) , ASCR, CD230, CJD, GSS, MGC26679, PRIP, PrP, PrP27-30, PrP33-35C, PrPc , CD230 antigen, major prion protein, prion protein, prion protein (p27-30) (Creutzfeld-Jakob disease Gerstmann-Strausler-Scheinker syndrome, fatal familial insomnia), prion protein PrP, prion-related protein
Protein Accession Number:
NP_000302.1
Protein Gi Number:
4506113
Ncbi Gene Id Number:
5621
Background Reference 1:
Ballerini C, Gourdain P, Bachy V, Blanchard N, Levavasseur E, Gregoire S, Fontes P, Aucouturier P, Hivroz C, Carnaud C. Functional Implication of Cellular Prion Protein in Antigen-Driven Interactions between T Cells and Dendritic Cells. J Immunol. 2006 Jun 15;176(12):7254-62.

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

45-123

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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