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Antibody used in WB on Human Muscle at 0.2-1 ug/ml.
Antibody used in WB on Human Muscle at 0.2-1 ug/ml.

PRKAA1 Antibody

CATALOG NUMBER: 30-953

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRKAA1.
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
PRKAA1 antibody can be used for detection of PRKAA1 by ELISA at 1:62500. PRKAA1 antibody can be used for detection of PRKAA1 by western blot at 1 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. XBL-10413 - Fetal Skeletal Muscle Tissue Lysate
Predicted Molecular Weight:
62 kDa
Purification:
Antibody is purified by peptide affinity chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PRKAA1 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PRKAA1
Additional Names:
PRKAA1, AMPK, AMPKa1, MGC33776, MGC57364
Protein Accession Number:
NP_996790
Protein Gi Number:
94557299
Ncbi Gene Id Number:
5562
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
PRKAA1 belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways.The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
Background Reference 1:
Hasumi, H., Gene 415 (1-2), 60-67 (2008).

FOR RESEARCH USE ONLY

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Disclaimer:
This product is for research use only.

CATALOG NUMBER:

30-953

List Size:
100 ul

List Price:

$519.00

Datasheet
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