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Western blot analysis of extracts of various cell lines, using PRKAB1 antibody (13-300) at 1:1000 dilution.<br/>Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.<br/>Detection: ECL Basic Kit.<br/>Exposure time: 15s.
Western blot analysis of extracts of various cell lines, using PRKAB1 antibody (13-300) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Mouse IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 15s.

PRKAB1 Antibody [1F9G6]

CATALOG NUMBER: 13-300

Clonality:
Monoclonal
Tested Applications:
ICC, IHC, IP, WB
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Mouse
Species Reactivity:
Human, Monkey, Mouse, Rat
Immunogen:
Recombinant protein of human PRKAB1
Conjugate:
Unconjugated
Tested Applications:
ICC, IHC, IP, WB
Application Note:
WB: 1:500 - 1:2000
IHC: 1:50 - 1:200
ICC: 1:20 - 1:50
IP: 1:50 - 1:200
Positive Control 1:
HeLa
Positive Control 2:
PC-12
Positive Control 3:
SH-SY5Y
Positive Control 4:
NCI-H460
Positive Control 5:
Jurkat
Positive Control 6:
NIH/3T3
Predicted Molecular Weight:
Observed: 38kDa
Purification:
Affinity purification
Clonality:
Monoclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at -20°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRKAB1
Additional Names:
PRKAB1, AMPK beta 1, AMPK subunit beta-1, AMPKb, AMPK, AMPK beta -1 chain, HAMPKb
Ncbi Gene Id Number:
5564
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a regulatory subunit of the AMP-activated protein kinase (AMPK). AMPK is a heterotrimer consisting of an alpha catalytic subunit, and non-catalytic beta and gamma subunits. AMPK is an important energy-sensing enzyme that monitors cellular energy status. In response to cellular metabolic stresses, AMPK is activated, and thus phosphorylates and inactivates acetyl-CoA carboxylase (ACC) and beta-hydroxy beta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved in regulating de novo biosynthesis of fatty acid and cholesterol. This subunit may be a positive regulator of AMPK activity. The myristoylation and phosphorylation of this subunit have been shown to affect the enzyme activity and cellular localization of AMPK. This subunit may also serve as an adaptor molecule mediating the association of the AMPK complex.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

13-300

List Size:
50 uL

List Price:

$335.00

Out of stock

Datasheet
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