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Western blot analysis of lysates from Hela, HepG2, RD cell line (from left to right), using PRKAG3 Antibody at 1:1000 at each lane.
Western blot analysis of lysates from Hela, HepG2, RD cell line (from left to right), using PRKAG3 Antibody at 1:1000 at each lane.

PRKAG3 Antibody

CATALOG NUMBER: 58-783

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Homology:
Predicted species reactivity based on immunogen sequence: Bovine, Mouse
Immunogen:
This PRKAG3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 426-454 amino acids from the C-terminal region of human PRKAG3.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
54 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKAG3
Additional Names:
5'-AMP-activated protein kinase subunit gamma-3, AMPK gamma3, AMPK subunit gamma-3, PRKAG3, AMPKG3
Protein Accession Number:
Q9UGI9
Protein Gi Number:
85681287
Ncbi Gene Id Number:
53632
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein encoded by this gene is a regulatory subunitof the AMP-activated protein kinase (AMPK). AMPK is a heterotrimerconsisting of an alpha catalytic subunit, and non-catalytic betaand gamma subunits. AMPK is an important energy-sensing enzyme thatmonitors cellular energy status. In response to cellular metabolicstresses, AMPK is activated, and thus phosphorylates andinactivates acetyl-CoA carboxylase (ACC) and beta-hydroxybeta-methylglutaryl-CoA reductase (HMGCR), key enzymes involved inregulating de novo biosynthesis of fatty acid and cholesterol. Thissubunit is one of the gamma regulatory subunits of AMPK. It isdominantly expressed in skeletal muscle. Studies of the pigcounterpart suggest that this subunit may play a key role in theregulation of energy metabolism in skeletal muscle. [provided byRefSeq].
Background Reference 1:
Jablonski, K.A., et al. Diabetes 59(10):2672-2681(2010)
Background Reference 2:
Jassim, G., et al. Pharmacopsychiatry (2010) In press :
Background Reference 3:
Crawford, S.A., et al. Diabetologia 53(9):1986-1997(2010)
Background Reference 4:
Ramanathan, L., et al. Protein Expr. Purif. 70(1):13-22(2010)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

58-783

List Size:
400 ul

List Price:

$495.00

Datasheet
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