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Western blot analysis in CEM cell line lysates (35ug/lane).
Western blot analysis in CEM cell line lysates (35ug/lane).

PRKAR1B Antibody

CATALOG NUMBER: 59-017

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
This PRKAR1B antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 55-84 amino acids from the N-terminal region of human PRKAR1B.
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
For WB starting dilution is: 1:1000
Predicted Molecular Weight:
43 kDa
Purification:
This antibody is purified through a protein A column, followed by peptide affinity purification.
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRKAR1B
Additional Names:
cAMP-dependent protein kinase type I-beta regulatory subunit, PRKAR1B
Protein Accession Number:
P31321
Protein Gi Number:
229463042
Ncbi Gene Id Number:
5575
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Cyclic AMP-dependent protein kinase A (PKA) is anessential enzyme in the signaling pathway of the second messengercAMP. Through phosphorylation of target proteins, PKA controls manybiochemical events in the cell including regulation of metabolism,ion transport, and gene transcription. The PKA holoenzyme iscomposed of 2 regulatory and 2 catalytic subunits and dissociatesfrom the regulatory subunits upon binding of cAMP.[supplied byOMIM].
Background Reference 1:
Silva, L.K., et al. Eur. J. Hum. Genet. (2010) In press :
Background Reference 2:
Liu, Y.J., et al. Obesity (Silver Spring) (2010) In press :
Background Reference 3:
Zhan, X., et al. Anal. Biochem. 354(2):279-289(2006)
Background Reference 4:
Gullingsrud, J., et al. Structure 14(1):141-149(2006)

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

59-017

List Size:
400 ul

List Price:

$495.00

Datasheet
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