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43-319 (0.3ug/ml) staining of Human Placenta (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
43-319 (5ug/ml) staining of paraffin embedded Human Spleen. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
43-319 (0.3ug/ml) staining of Human Placenta (A) and Rat Brain (B) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PRKCB Antibody

CATALOG NUMBER: 43-319

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog
Immunogen:
The immunogen for this antibody is: C-DKEFTRQPVELT
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:128000.Western Blot:Approx 80kDa band observed in lysates of Human Placenta and Rat Brain (calculated MW of 76.9kDa according to NP_997700.1). Recommended concentration: 0.1-0.3ug/ml.Immunohistochemistry:In paraffin embedded Human Spleen shows nucleolar staining in some splenocytes. Recommended concentration, 1-2ug/ml.Additional validation: This antibody has been successfully used in the following paper: Sikorski et al. (2018) PMID: 30377371.
Specificity:
This antibody is expected to recognize isoform 1 (NP_997700.1) only.
Predicted Molecular Weight:
Approx 80kDa
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PRKCB
Additional Names:
PRKCB, protein kinase C, beta, MGC41878, PKC-beta, PKCB, PRKCB1, PRKCB2, OTTHUMP00000162377, OTTHUMP00000162378, PKC-B, protein kinase C beta type, protein kinase C, beta 1 polypeptide
Protein Accession Number:
NP_997700.1
Protein Gi Number:
20127450
Ncbi Gene Id Number:
5579
Background Reference 1:
Sheng YJ, Gao JP, Li J, Han JW, Xu Q, Hu WL, Pan TM, Cheng YL, Yu ZY, Ni C, Yao S, He CF, Liu YS, Li Y, Ge HM, Xiao FL, Sun LD, Yang S, Zhang XJ.Follow-up study identifies two novel susceptibility loci PRKCB and 8p11.21 for systemic lupus erythematosus.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

43-319

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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