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42-178 (0.1ug/ml) staining of Human Adipose cell llysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-178 (0.3ug/ml) staining of Mouse Ovary (A) and Rat Uterus (B) lysate (35ug protein in RIPA buffer). Detected by chemiluminescence.
42-178 (3.75ug/ml) staining of paraffin embedded Human Uterus. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.
42-178 (0.1ug/ml) staining of Human Adipose cell llysate (35ug protein in RIPA buffer). Detected by chemiluminescence.

PRKCDBP Antibody

CATALOG NUMBER: 42-178

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human, Mouse, Rat
Homology:
Expected Species Reactivity based on sequence homology: Dog, Cow
Immunogen:
The immunogen for this antibody is: C-RRTGLQKVQSLR
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:32000.Western Blot:Approx 38kDa band observed in Human Adipose, in Mouse Ovary and Rat Uterus and in NIH3T3 lysates (calculated MW of 27.7kDa according to Human NP_659477.2, of 27.8kDa according to Mouse NP_082720.1 and of 27.9kDa according to Rat NP_604444.1). The observed molecular weight corresponds to earlier findings in literature with different antibodies (Bastiani M, J Cell Biol. 2009 Jun 29;185(7):1259-73. PMID: 19546242 ). Recommended concentration: 0.1-1ug/ml. Primary incubation was 1 hour.Immunohistochemistry:Paraffin embedded Human Uterus. Recommended concentration: 3.75ug/ml.
Positive Control 1:
Cat. No. XBL-11048 - Human Adipose Tissue Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PRKCDBP
Additional Names:
PRKCDBP, protein kinase C, delta binding protein, HSRBC, MGC20400, SRBC, sdr-related gene product that binds to c-kinase, cavin-3
Protein Accession Number:
NP_659477.2
Protein Gi Number:
47132587
Ncbi Gene Id Number:
112464
Background Reference 1:
McMahon KA, Zajicek H, Li WP, Peyton MJ, Minna JD, Hernandez VJ, Luby-Phelps K, Anderson RG. SRBC/cavin-3 is a caveolin adapter protein that regulates caveolae function. The EMBO journal 2009 Apr 28 (8): 1001-15.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

42-178

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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