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Western blot analysis of extracts of HeLa cells, using PRKCSH antibody (19-255).<br/>Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.<br/>Lysates/proteins: 25ug per lane.<br/>Blocking buffer: 3% nonfat dry milk in TBST.
Western blot analysis of extracts of HeLa cells, using PRKCSH antibody (19-255).
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.

PRKCSH Antibody

CATALOG NUMBER: 19-255

Clonality:
Polyclonal
Tested Applications:
WB
Host Species:
Rabbit
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human
Immunogen:
A synthetic Peptide of human PRKCSH
Conjugate:
Unconjugated
Tested Applications:
WB
Application Note:
WB: 1:500 - 1:1000
Positive Control 1:
HeLa
Predicted Molecular Weight:
Observed: 72kDa
Purification:
Affinity purification
Clonality:
Polyclonal
Isotype:
IgG
Physical State:
Liquid
Buffer:
PBS with 0.02% sodium azide, pH7.3.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C. Avoid freeze / thaw cycles.
Ncbi Official Symbol:
PRKCSH
Additional Names:
AGE-R2, G19P1, GIIB, PCLD, PCLD1, PKCSH, PLD1, VASAP-6glucosidase 2 subunit beta, AGE-binding receptor 2, advanced glycation end-product receptor 2, glucosidase II subunit beta, hepatocystin, protein kinase C substrate 60.1 kDa protein heavy chain, protein kinase C substrate, 80 Kda protein
Ncbi Gene Id Number:
5589
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
This gene encodes the beta-subunit of glucosidase II, an N-linked glycan-processing enzyme in the endoplasmic reticulum. The encoded protein is an acidic phosphoprotein known to be a substrate for protein kinase C. Mutations in this gene have been associated with the autosomal dominant polycystic liver disease. Alternative splicing results in multiple transcript variants.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

19-255

List Size:
100 uL

List Price:

$423.00

Datasheet
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