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46-225 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.
46-225 (1ug/ml) staining of A549 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

PRL-1 Antibody

CATALOG NUMBER: 46-225

Clonality:
Polyclonal
Tested Applications:
ELISA, WB
Host Species:
Goat
Species Reactivity:
Human
Conjugate:
Unconjugated
Specifications
Host Species:
Goat
Species Reactivity:
Human
Homology:
Expected Species Reactivity based on sequence homology: Mouse, Rat, Dog
Immunogen:
The immunogen for this antibody is: KDSNGHRNNCCIQ
Conjugate:
Unconjugated
Tested Applications:
ELISA, WB
Application Note:
Peptide ELISA: antibody detection limit dilution 1:2000.Western Blot:Approx 21kDa band observed in lysates of cell lines A549 and HeLa (calculated MW of 19.8kDa according to NP_003454.1). Recommended concentration: 1-3ug/ml.
Positive Control 1:
Cat. No. 1203 - A549 Cell Lysate
Purification:
Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin.Aliquot and store at -20°C. Minimize freezing and thawing.
Concentration:
500 ug/mL
Storage Conditions:
Aliquot and store at -20°C. Minimize freezing and thawing.
Ncbi Official Symbol:
PTP4A1
Additional Names:
PTP4A1, PRL-1, protein tyrosine phosphatase type IVA, member 1, HH72, PRL1, PTP4A2, PTPCAAX1, PTP(CAAX1), Protein tyrosine phosphatase IVA1, RP5-1148A21.2, DKFZp779M0721, protein tyrosine phosphatase type IVA protein 1
Protein Accession Number:
NP_003454.1
Protein Gi Number:
4506283
Ncbi Gene Id Number:
7803
Background Reference 1:
Peng Y, Genin A, Spinner NB, Diamond RH, Taub R. The gene encoding human nuclear protein tyrosine phosphatase, PRL-1. Cloning, chromosomal localization, and identification of an intron enhancer. J Biol Chem 1998 Jul 3;273(27):17286-95

FOR RESEARCH USE ONLY

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Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

46-225

List Size:
0.1 mg

List Price:

$495.00

Datasheet
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