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Western blot analysis of anti- in Hela cell line lysates (35ug/lane)
Western blot analysis in mouse Neuro-2a cell line lysates (35ug/lane).This demonstrates the PRMT1 antibody detected the PRMT1 protein (arrow).
Formalin-fixed and paraffin-embedded human breast carcinoma tissue reacted with , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Western blot analysis of anti- in Hela cell line lysates (35ug/lane)

PRMT1 Antibody

CATALOG NUMBER: 55-010

Clonality:
Polyclonal
Tested Applications:
IHC-P, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse
Homology:
Predicted species reactivity based on immunogen sequence: Rat
Immunogen:
This PRMT1 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 317-346 amino acids from the C-terminal region of human PRMT1.
Conjugate:
Unconjugated
Tested Applications:
IHC-P, WB
Application Note:
For WB starting dilution is: 1:1000
For IHC-P starting dilution is: 1:10~50
Predicted Molecular Weight:
42 kDa
Purification:
This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis
Clonality:
Polyclonal
Isotype:
Rabbit Ig
Physical State:
Liquid
Buffer:
Supplied in PBS with 0.09% (W/V) sodium azide.
Concentration:
batch dependent
Storage Conditions:
Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
Ncbi Official Symbol:
PRMT1
Additional Names:
Protein arginine N-methyltransferase 1, 211-, Histone-arginine N-methyltransferase PRMT1, Interferon receptor 1-bound protein 4, PRMT1, HMT2, HRMT1L2, IR1B4
Protein Accession Number:
Q99873
Protein Gi Number:
161789011
Ncbi Gene Id Number:
3276
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
Arginine methylation is an irreversible post translational modification which has only recently been linked to protein activity. At least three types of PRMT enzymes have been identified in mammalian cells. These enzymes have been shown to have essential regulatory functions by methylation of key proteins in several fundamental areas. These protein include nuclear proteins, IL enhancer binding factor, nuclear factors, cell cycle proteins, signal transduction proteins, apoptosis proteins, and viral proteins. The mammalian PRMT family currently consists of 7 members that share two large domains of homology.
Background Reference 1:
Zhang, X., et al., EMBO J. 19(14):3509-3519 (2000).
Background Reference 2:
Scorilas, A., et al., Biochem. Biophys. Res. Commun. 278(2):349-359 (2000).
Background Reference 3:
Scott, H.S., et al., Genomics 48(3):330-340 (1998).
Background Reference 4:
Abramovich, C., et al., EMBO J. 16(2):260-266 (1997).

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
Optimal dilutions/concentrations should be determined by the end user. The information provided is a guideline for product use. This product is for research use only.

CATALOG NUMBER:

55-010

List Size:
400 ul

List Price:

$495.00

Datasheet
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