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Antibody used in WB on Human Jurkat 0.625 ug/ml.
Antibody used in WB on Human, Mouse at 1:1000.
Antibody used in IHC on Human liver at 4.0-8.0 ug/ml.
Antibody used in WB on Human Jurkat 0.625 ug/ml.

PRMT2 Antibody

CATALOG NUMBER: 29-206

Clonality:
Polyclonal
Tested Applications:
ELISA, IHC, WB
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Conjugate:
Unconjugated
Specifications
Host Species:
Rabbit
Species Reactivity:
Human, Mouse, Rat
Immunogen:
Antibody produced in rabbits immunized with a synthetic peptide corresponding a region of human PRMT2.
Conjugate:
Unconjugated
Tested Applications:
ELISA, IHC, WB
Application Note:
PRMT2 antibody can be used for detection of PRMT2 by ELISA at 1:1562500. PRMT2 antibody can be used for detection of PRMT2 by western blot at 0.625 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000.
Positive Control 1:
Cat. No. 1205 - Jurkat Cell Lysate
Predicted Molecular Weight:
48 kDa, 49 kDa
Purification:
Antibody is purified by protein A chromatography method.
Clonality:
Polyclonal
Physical State:
Liquid
Buffer:
Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.
Concentration:
batch dependent
Storage Conditions:
For short periods of storage (days) store at 4°C. For longer periods of storage, store PRMT2 antibody at -20°C. As with any antibody avoid repeat freeze-thaw cycles.
Ncbi Official Symbol:
PRMT2
Additional Names:
PRMT2, HRMT1L1
Protein Accession Number:
NP_001526
Protein Gi Number:
46255047
Ncbi Gene Id Number:
3275
User Note:
Optimal dilutions for each application to be determined by the researcher.
Background:
The protein arginine methyltransferases (PRMTs) include a family of proteins with related putative methyltransferase domains that modify chromatin and regulate cellular transcription. PRMT2 inhibits NF-kappaB-dependent transcription and promotes apoptosis and it exerts this effect by blocking nuclear export of IkappaB-alpha through a leptomycin-sensitive pathway, increasing nuclear IkappaB-alpha and decreasing NF-kappaB DNA binding. PRMT2 also rendered cells susceptible to apoptosis by cytokines or cytotoxic drugs, likely due to its effects on NF-kappaB.
Background Reference 1:
Qi, C., (2002) J. Biol. Chem. 277 (32), 28624-28630.

FOR RESEARCH USE ONLY

For additional information, visit ProSci’s Terms & Conditions Page.

Disclaimer:
This product is for research use only.

CATALOG NUMBER:

29-206

List Size:
100 ul

List Price:

$473.00

Datasheet
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